The Cell Ops open day is an opportunity for you to discover the great in-house services available to researchers on campus. There will be tours, games and a chance to discuss your projects and how we can help you to complete them.
Come and meet the teams on Thursday 29th November 1pm-4pm in the Sulston building. We will have someone at Sulston reception to let you in, no need to worry about access.
High-throughput gene editing: F334
Bespoke gene editing: F334
The Cellular Generation and Phenotyping Core Facility is a large cell biology core facility consisting of about 32 scientists from a variety of scientific backgrounds. We work closely with faculty to carry out large scale cell biology projects to help further the institutes scientific aims. We work across all areas of cell biology with particular expertise in stem cells, organoid production and genome wide CRISPR screening.
The Cytometry Core Facility is a dedicated scientific service offering investigators state of art instrumentation and expertise in flow cytometry.
- Researcher operated cell analysers,
- Staff and researcher operated cell sorters
- Experimental design and consultation
- Training on cell analysers, cell sorters and in data analysis
High-throughput Gene Editing
We have established highly efficient methods that take advantage of CRISPR-Cas9 technology to generate constitutive knockouts in iPS cells at scale. The scope of our service to faculty includes the provision of experience, advice and assistance in the design and implementation of routine gene editing projects in iPS cells.
Bespoke Gene Editing
We use CRISPR/Cas9 techniques to generate iPS cell lines involving more complex alleles.
- Single nucleotide polymorphisms (SNPs) e.g. to replicate or repair disease alleles
- Conditional alleles e.g. Cre-dependent FLIP alleles, chemically or optically induced degrons
- Tagged alleles e.g. fluorescent reporters, affinity tags, degrons
- Multiple compound mutations e.g. sets of mutations in a gene family
We look forward to meeting you on the 29th November